Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production

Table 2 Experimental vs Response surface predictions and statistical values calculated from fermentations used for model validation

initial GLC:YE g/L		75:20		80:40		115:45		%Error
		Exp.	Model	Exp.	Model	Exp.	Model	Average
Max biomass	[g/L]	9.30	8.40	14.30	16.03	19.76	18.05	7.18
Consumed GLC	[g/L]	70.03	70.70	80.11	78.19	115.76	113.84	1.18
Final SA	[g/L]	24.62	22.13	33.19	26.62	31.84	32.96	7.88
Final AC	[g/L]	5.25	6.45	0.00	0.85	4.90	8.73	35.70
μ _max	[h ⁻¹]	0.62	0.73	0.78	0.77	0.55	0.65	8.56
\(q_{glc}^{exp}\)	[g/Lh]	0.89	1.17	0.93	0.97	0.73	0.99	17.03
\(q_{sa}^{exp}\)	[g/Lh]	0.36	0.42	0.32	0.28	0.24	0.24	7.35
\(q_{glc}^{sta}\)	[g/Lh]	0.33	0.32	0.38	0.39	0.41	0.42	2.07
\(q_{sa}^{sta}\)	[g/Lh]	0.15	0.13	0.18	0.18	0.15	0.15	2.88
Y _ps	[g/g]	0.41	0.36	0.35	0.29	0.34	0.23	14.49
Y _px	[g/g]	0.59	0.59	0.42	0.38	0.44	0.38	5.68

ISSN: 1752-0509