Fig. 7

Bcd orientation and spacing does not rescue s272 Δ gt ΔKr. For each sequence, the binding structure structure is shown (left). Height of bars is proportional to LLR of binding for each motif. A subset of motifs are shown. Binding sites for all factors considered in this work are included in Additional file 1: Figures S5-S15. We also show FISH for lacZ driven by each of the sequences (right). a s272 Δ gt ΔKr with the second Bcd motif orientation reversed. The resulting enhancer does does not drive expression. b s272 Δ gt ΔKr with the 5 bp of inter-motif spacer removed. The resulting enhancer does not drive expression. c s272 Δ gt ΔKr with the second Bcd motif orientation reversed and Hb site deleted. The resulting enhancer does not drive expression