Fig. 1From: Cooperative binding mitigates the high-dose hook effectBinding of ligands A, B to a bivalent linker protein L. a Low linker concentration: availability of L limits the formation of total complexes (LAB, in colour). b Linker concentration on the order of ligand concentration: Formation of fully formed complex (LAB) reaches its maximum. c Concentration of linker L much higher than that of A or B: partially bound forms prevail, and formation of fully formed complex (LAB) goes down in absolute termsBack to article page