13C metabolic flux analysis shows that resistin impairs the metabolic response to insulin in L6E9 myotubes

BMC Systems Biology

Table 1 Biochemical parameters in L6E9 myotubes after different incubations with resistin and/or insulin

	Incubation condition
	Ins-		Ins+
	Res-	Res+	Res-	Res+
Glucose consumption (mM)	0.50±0.04	0.61±0.06*	0.78±0.03**	0.70±0.09
Lactate production (mM)	0.48±0.01	0.56±0.02**	0.75±0.06**	0.79±0.02^†
Glycogen (µg glucose/mg prot)	9.88±1.99	9.97±0.98	11.34±0.59	11.62±1.21
Glucose-6-phosphate (nmol/mg prot)	0.92±0.14	1.44±0.28	2.30±0.29**	2.10±0.19^†

L6E9 myotubes were treated (Res+) or not treated (Res-) with 100 nM resistin for 8 h and then incubated for 6 h in the absence (Ins-) or presence (Ins+) of 100 nM insulin. Glucose consumption, lactate production, and intracellular contents of glycogen and glucose-6-phosphate were determined as described in the Methods section. Results are the mean±standard deviation (n=3). Units of glucose consumption and lactate production refer to the volume of the incubation medium. (*) indicates p<0.05 and (**) p<0.01 compared with the condition of neither resistin nor insulin. (†) indicates p<0.05 compared with the L6E9 myotubes treated only with resistin.

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