Figure 2

Gene expression of enzymes related to pyruvate homeostasis. L6E9 myotubes were treated (Res+) or not treated (Res-) with 100 nM resistin for 8 h and then incubated for 6 h with 10 mM glucose in the absence (Ins-) or presence (Ins+) of 100 nM insulin. RT-qPCR monitoring of mRNA levels of pyruvate carboxylase (Pc) (A), pyruvate kinase muscle isozyme (Pkm2) (B), pyruvate dehydrogenase E1 alpha 1 (Pdha1) (C), pyruvate dehydrogenase kinase isozyme 2 (Pdk2) (D), pyruvate dehydrogenase kinase isozyme 4 (Pdk4) (E), cytosolic NADP-isocitrate dehydrogenase (Idh1) (F), NADP-dependent malic enzyme (Me1) (G) and cytosolic phosphoenolpyruvate carboxykinase 1 (Pck1) (H) are shown. Results from three experiments are expressed as the mean±S.E.M. (*) indicates p<0.05 and (**) p<0.01 compared with the condition of neither resistin nor insulin. (#) indicates p<0.05 and (##) p<0.01 compared with L6E9 myotubes treated only with resistin.