Optimization and model reduction in the high dimensional parameter space of a budding yeast cell cycle model

BMC Systems Biology

Table 5 The 20 most critical model parameters

Parameter name	Percent of losses
Total amount of Cdc14	3.14
SPN synthesis rate	2.81
Total amount of Esp1	2.60
Total amount of Net1	2.57
Degradation rate of Cdc20	2.53
PPX inactivation by Esp1	2.51
Efficiency of Cdc14-Net1 complex (RENT) formation	2.50
Time scale for protein activation	2.41
Net1 phosphorylation by Clb2	2.30
Total amount of Mcm1	2.25
Transcriptional activation of CDC20 by Mcm1	2.17
Transcriptional activation of CLB2 by Mcm1	2.06
Sigmoidicity of protein activation	1.99
Degradation rate of Swi5	1.99
CKI phosphorylation rate	1.96
Cdh1 inactivation rate	1.83
Total amount of SBF	1.79
Clb2 degradation by active Cdc20	1.76
Polo activation by Clb2	1.67
Synthesis rate of Bck2	1.66

Most critical model parameters that had the largest effects on the objective function upon perturbations. Criticality decreases from top to bottom.

ISSN: 1752-0509