Figure 3

Four relevant paths for acetate overflow calculated by iCFP. A, First path; B, Second path; C, Third path; D, Fourth path. Highly/over expressed reactions are represented by solid lines, Medium expressed/invariant reactions by dashed lines and Lowly/down expressed reactions by dotted lines. Metabolites: Ac, Acetate; AcAld, Acetaldehyde; AcCoA, Acetyl-CoA; AcG5P, N-Acetyl-L-glutamyl 5-phosphate; AcG5sA, N-Acetyl-L-glutamate 5-semialdehyde; AcGlu, N-Acetyl-L-glutamate; AcP, Acetyl phosphate; D-Glc, D-Glucose; L-Asp, L-Aspartate; L-AspsA, L-Aspartate 4-semialdehyde; L-Hom, L-Homoserine; L-PHom, O-Phospho-L-homoserine; L-Thr, L-Threonine; OAA, Oxaloacetate; Pyr, Pyruvate. Reactions: ACALD, acetaldehyde dehydrogenase (acetylating); ACGK, acetylglutamate kinase; ACGS, N-acetylglutamate synthase; ACKr, acetate kinase; AGPR, N-acetyl-g-glutamyl-phosphate reductase; ALDD2x, aldehyde dehydrogenase (acetaldehyde, NAD); ASAD, aspartate-semialdehyde dehydrogenase; ASPK, aspartate kinase; ASPTA, aspartate transaminase; HSDy, homoserine dehydrogenase (NADPH); HSK, homoserine kinase; NACODA, N-acetylornithine deacetylase; PDH, pyruvate dehydrogenase; PTA, phosphotransacetylase; THRAi, Threonine aldolase; THRS, threonine synthase.