Figure 3
The switch design example of synthetic genetic transistor. For switch design example of synthetic genetic transistor, a desired I/O switch response is computed by (15) as shown in red line. The most adequate promoter-RBS component c3 and aTc concentration I aTc to fit the desired I/O switch response are searched as {c3, I aTc } = {T1, 0 ng/ml} by minimizing J S (c3, I aTc ) in (16) from the corresponding promoter-RBS library Lib Tet and concentration range of inducer I aTc . The green points are the experimental results, and the error bars are the standard deviations. The green line is the estimated I/O switch response based on experimental data. Obviously, the I/O response of the designed genetic switch could match the desired I/O switch response quite well.