Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling

Table 2 Overview of quantification methods used for intracellular metabolite determination.

Metabolite	Detector	Calibration type	Concentration in standard stock [μ M]
3PG	ECD	QOff	25
ADP	UV	LOff	200
AMP	UV	XLOff	100
ATP	UV	LOff	400
CDP	UV	QOff	25
*cis* -Aconitate	MS	XQuad	25
Citrate	ECD	QOff	250
CMP	UV	LOff	25
CTP	UV	LOff	40
Fructose-1,6-bP	ECD	XQOff	228
Fructose-6-P	MS	QOff	35
Fumarate	UV	XLOff	50
GDP	UV	LOff	40
Glucose-6-P	MS	XQOff	35
GMP	MS	XQuad	25
GTP	UV	LOff	79
Isocitrate	MS	XQuad	25
Malate	MS	Quad	200
PEP	MS	XQuad	25
Pyruvate	MS	Quad	200
Ribose-5-P	MS	XQuad	25
Succinate	MS	XQOff	200
UDP	UV	LOff	25
UDP-Glc	MS	XQOff	70
UDP-NAc-Gal	UV	XLOff	60
UDP-NAc-Glc	UV	XLOff	200
UMP	MS	XLOff	75
UTP	UV	Lin	200
α -Ketoglutarate	MS	XQOff	100

Lin: Polynomial 1. Ord. forced through origin
LOff: Polynomial 1. Ord. NOT forced origin
XLOff: Polynomial 1. Ord. NOT forced origin, weighted (1/concentration)
Quad: Polynomial 2. Ord. forced through origin
QOff: Polynomial 2. Ord. NOT forced through origin
XQuad: Polynomial 2. Ord. forced through origin, weighted (1/concentration)
XQOff: Polynomial 2. Ord. NOT forced through origin, weighted (1/concentration)

ISSN: 1752-0509