Figure 5

Statistical analysis of phagocytic cup shapes. (A) Experimental cup variability, defined in Figure 4, for 20-40% engulfed particles, for COS-7 cells transfected with wild-type FcγR (WT-FcγR) or signaling-dead mutant (Y282F/Y298F-FcγR) in DMEM medium, as well as WT-FcγR cells treated with 0.2 μ M cytochalasin D (WT-FcγR+CytoD). The data points represent the variability averaged over all the phagocytic cups measured for given condition (receptor type and treatment) and range of engulfment. Error bars represent the statistical standard error of the cup variability. Student's t-test: p-values are (*) 0.0002 and (**) 0.0003. (Inset) Theoretical cup variability calculated from corresponding model simulations. Data points and error bars represent respectively the statistical average and standard error of the cup variability over several simulations. Student's t-test (*): p-value is less than 0.0001. (B) Experimental cup variability for 40-60% engulfed particles. (Inset) Theoretical cup variability calculated from corresponding model simulations. Student's t-test (*): p-value is given less than 0.0001. (C) Repartitioning of regular and variable cups for different experimental conditions, i.e. wild-type receptor (WT-FcγR), signaling-dead mutant (Y282F/Y298F-FcγR) and cytochalasin-D treated cells (WT-FcγR+CytoD), for engulfment between 20 and 40%. The numbers indicate the contribution (in %) of the three conditions in the overall sample of regular (left) and variable (right) cups.