shRNA library screening of Abl kinase-induced cell detachment. (A) Schematic outline of the experimental setup for the identification of effectors in AblPP-induced cell detachment. Details provided in Methods. (B) Percentage of detached cells after each round of AblPP induction. AblPP cells before (NI) and after (1st and 2nd) shRNA library transduction was subjected to AblPP-induced cell detachment. Following the 2nd round of AblPP induction, AblPP cells did not detach anymore. (C) AblPP was induced to similar levels and exhibited similar kinase activity during each round of AblPP induction.