A mathematical model of iron import and trafficking in wild-type and Mrs3/4ΔΔ yeast cells

BMC Systems Biology

Table 1 Growth rates, iron concentrations, and import rates in ΔΔ and WT cells grown under different nutrient conditions. [N] refers to the μM concentration of iron in the respiring medium, as described [34]. The untreated medium was assumed to contain 1 μM of endogenous iron. For each entry, the top number is datum or data-based estimates (R_…-dat) and the bottom number is the corresponding simulated value (R_…-sim). Concentrations are in units of μM, rates are in units of μM/hr., and α_cell is in units of hr.^− 1. Data for α_cell and [Fe_cell] have been published [34] whereas [Fe_cyt], [Fe_mit], and [Fe_vac] were estimated as described in the text

[N]	α_cell	[Fe_cell]	R _cell	f_cyt⋅[Fe_cyt]	f_mit ⋅[Fe_mit]	f_vac ⋅[Fe_vac]	R _cyt - R _mit - R _vac	R _mit	R _vac
WT
1	0.18	120	22	79	41	0	18	9.2	0
1	0.18	60	10	22	22	16	5.0	4.9	3.7
2	0.18	200	37	75	43	82	17	9.9	19
2	0.19	190	35	76	35	81	18	8.4	19
11	0.20	480	97	200	56	220	52	14	56
11	0.20	810	160	180	66	560	45	17	140
41	0.20	880	180	310	69	500	77	18	130
41	0.20	900	180	180	69	640	46	18	160
ΔΔ
1	0.06	360	22	110	69	180	8.1	5.2	14
1	0.05	540	27	240	74	230	13	3.9	12
2	0.068	680	46	130	69	480	11	5.9	41
2	0.069	1100	90	380	77	660	33	6.6	58
11	0.15	2200	320	280	72	1800	52	13	340
11	0.15	2300	360	280	71	1900	53	13	360
41	0.20	3900	800	230	74	3600	59	19	920
41	0.19	2000	390	230	61	1800	54	14	410

ISSN: 1752-0509