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Fig. 6 | BMC Systems Biology

Fig. 6

From: The synthetic histone-binding regulator protein PcTF activates interferon genes in breast cancer cells

Fig. 6

RT-qPCR analysis of gene expression in stable, transgenic PcTF-expressing cells. a SfiI-flanked PcTF or PcΔTF constructs (top) were cloned into the pSBtet-GP expression vector (bottom), resulting in the replacement of the luciferase reporter with fusion protein ORFs. b Fluorescence microscopy of the MCF7-PcTF transgenic cell line. c Time course RT-qPCR for PcTF. d Time course RT-qPCR for select genes. For all RT-qPCR experiments n = two cDNA libraries from independent transfections or dox treatments. FC, fold change relative to “no dox” controls, calculated as double delta Cp (see Methods)

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