Fig. 1
From: Reconstruction of a genome-scale metabolic model for Actinobacillus succinogenes 130Z
Metabolic functional annotation of CDSs. The implemented framework uses an automated annotation tool coupled with manual curation procedures that allows the assignment of metabolic functions to CDSs of a particular genome. The pipeline comprises five main steps: (1) homology search CDSs against databases like UniProt; (2) computation of functional scores based on similarity; (3) assignment of putative metabolic function(s); (4) manual curation of functions; and finally (5) association of metabolic reactions