Fig. 1

Overview of reconstructing human structural interactome (hDiSNet) using 3D–domain interolog mapping. a 3D–domain interolog mapping infers human PPIs through 60,618 three-dimensional (3D) structural complexes and complete genome database with 6,352,363 protein sequences across 2274 species using the 3D–template 1ev2 complex as the example. We totally infer the 19,239 PPIs in 5177 proteins in the reconstructed human structural interactome (hDiSNet) from 60,618 structural templates. b The largest sub-network of hDiSNet with 2051 proteins and 11,534 PPIs can be grouped into nine major cellular processes, including extracellular proteolysis (yellow), nucleic acid metabolic (red), cellular protein metabolic (brown), nuclear part cell cycle (pink), membrane signal transduction (purple), cytoplasm part signal transduction (cyan), cytoplasm part signal protein transport (green), cytoplasmic proteolysis (orange), and cytoskeletal part organelle organization (blue). c The FGFR (EGFR)-MAPK pathway in hDiSNet. The node sizes of circle (proteins) and box (cancer-related proteins) denote the numbers of mutations recorded in OMIM database. The colored borders of nodes indicate the numbers of diseases associated proteins. The colored nodes indicate the ratios of mutations in the contacting and non-contacting residues. d Detailed atomic interactions of FGFR2-FGF2 and the MSA of contacting residues across multiple species. The contacting residues are colored according to their types: for forming hydrogen bonds (green), conserved (orange), both for forming hydrogen bonds and conserved (yellow), and for forming van der Waals force (gray)