Skip to main content

Table 4 Sensitivity parameters assessed using FBA - Scaled shadow prices, Logarithmic sensitivity and Maximum reduced costs

From: A scalable metabolite supplementation strategy against antibiotic resistant pathogen Chromobacterium violaceum induced by NAD+/NADH+ imbalance

 

Metabolite

Maximum Precursor Yield (M/Glc)

Shadow price in BOF (dX/dM)

Coefficient in BOF (dM)

Scaled shadow price (SSP)

Logarithmic sensitivity (LS)

WT

NADPH

0.004

−0.0079

13.028

−1.36E-04

−0.1024

NADH

0.0061

−0.003

−3.547

−7.95E-05

0.0107

ATP

0.0056

−0.0097

59.81

−2.37E-04

−0.5784

ChlR

NADPH

0.0034

−0.0054

13.028

−5.56E-05

−0.07

NADH

0.0101

2.09E-18

−3.547

6.48E-20

−7.40E-18

ATP

0.0034

−0.0108

59.81

−1.11E-04

−0.643

StrpR

NADPH

0.0009

−0.008

13.028

−1.11E-05

−0.1043

NADH

0.0017

−0.0031

−3.547

−8.04E-06

0.0109

ATP

0.0013

−0.0099

59.81

−1.93E-05

−0.5895

 

Reaction ID

Reaction Name

WT

CHLR

STRPR

Maximum Scaled Reduced Cost

AKGDH

2-Oxoglutarate dehydrogenase

0

−8.11E-07

0

EX_o2(e)

Oxygen Exchange

0

1.106

0.683

ICL

Isocitrate lyase

0

0

−4.86E-07

PGL

6 - Phosphogluconolactonase

0

−8.11E-07

−4.86E-07

SUCCt3

Succinate transporter

0

−8.11E-07

−4.86E-07

PPNDH

Prephenate dehydratase

0

−8.11E-07

0

GLCt2

Glucose Transporter

0

−8.11E-07

−4.86E-07

Rxnvio8

Reaction 8 of Violacein Synthesis

0

−6.56E-08

−2.81E-08