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Fig. 1 | BMC Systems Biology

Fig. 1

From: Membrane potential independent transport of NH3 in the absence of ammonium permeases in Saccharomyces cerevisiae

Fig. 1

Relative specific growth rate in micro-titer plate (μMTP) of (a) IME169 (reference strain) and (b) IMZ351 (Δmep) at different pH and extracellular NHX-concentrations, pH = 5 (blue), pH = 6 (red) and pH = 7 (green) in synthetic medium with glucose supplemented with Tween-80 (420 mg/L) and ergosterol (10 mg/L). c Relative specific growth rates in micro-titer plate (μMTP) of IMZ351 at different NH3-concentrations irrespective of extracellular pH. Growth rates were determined from exponentially growing cells cultured in 100 μL synthetic medium in 96 well plates with OD660 measurements taken every 15 min. The SO4 concentration was kept constant at 38 mM by supplementation with K2SO4. Data are presented as averages and standard deviations of duplicate experiments, relative to the average growth rate of IME169 at pH = 5, with 76 mM NH4 + (μmax = 0.21 h−1). The continuous blue line represents an apparent permeability coefficient of 0.0115 m/h (0.32 10−3 cm/s), calculated using least squares in the linear region of the experimental data (R2 = 0.73); the discontinuous black line shows the trend of the growth rate if an apparent permeability coefficient of 1.728 m/h (48 10−3 cm/s) is assumed [17]

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