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Table 2 Medium metabolite and amino acid secretion and uptake comparison between uninfected control (Ctr) red blood cell (RBC) cultures versus Plasmodium falciparum (Pf) infected (Inf) RBC cultures

From: Metabolic host responses to malarial infection during the intraerythrocytic developmental cycle

Metabolite

C t=0 h

ΔCtrt=48 h

ΔInft=48 h

ΔPf Inf

Secretion(+)/Uptake(−)

mM

mM

mM

mM

RBC

Pf

Glucose

1.11 10+1

−5.07 10−1

−9.97 10+0

−9.5 10+0

– – –

– – – –

Myo-Inositol

1.94 10−1

−7.29 10−2

−7.28 10−2

8.7 10−5

– –

– –

Hypoxanthine

1.00 10−1

−1.87 10−2

−8.09 10−2

−6.2 10−2

– –

– – –

Choline

2.15 10−2

4.05 10−3

−1.47 10−3

−5.5 10−3

+

–

Nicotinamide

8.19 10−3

0.00 10+0

−4.95 10−4

−5.0 10−4

â—‹

–

p-aminobenzoate

7.29 10−3

0.00 10+0

0.00 10+0

0.0 10+0

â—‹

â—‹

Pyridoxine

4.86 10−3

−2.73 10−3

−2.73 10−3

0.0 10+0

–

–

Thiamine

2.96 10−3

2.18 10−3

1.99 10−3

−1.9 10−4

+

+

Riboflavin

5.31 10−4

0.00 10+0

−9.35 10−5

−9.3 10−5

â—‹

–

Pantothenate

5.25 10−4

0.00 10+0

−1.25 10−4

−1.2 10−4

â—‹

–

Amino acid

C t=0 h

ΔCtrt=48 h

ΔInft=48 h

ΔPf Inf

Secretion(+)/Uptake(−)

mM

mM

mM

mM

RBC

Pf

Arginine

1.148

0.012

0.026

1.5 10−2

+

+

Asparagine

0.378

0.074

0.036

−3.9 10−2

+

+

Aspartate

0.150

0.213

0.200

−1.2 10−2

++

++

Glutamate

0.136

0.848

0.726

−1.2 10−1

+++

+++

Glutamine

2.053

0.479

0.518

3.8 10−2

+++

+++

Histidine

0.097

0.090

0.279

1.9 10−1

+

++

(Iso)Leucine

0.762

0.324

0.647

3.2 10−1

+

+++

Methionine

0.101

0.021

0.052

3.1 10−2

+

+

Phenylalanine

0.091

0.143

0.268

1.2 10−1

+

++

Proline

0.174

0.149

0.280

1.3 10−1

+

++

Serine

0.285

0.149

0.210

6.2 10−2

+

++

Tryptophan

0.024

0.011

0.039

2.9 10−2

+

+

Tyrosine

0.110

0.106

0.140

3.4 10−2

+

++

Valine

0.171

0.191

0.491

3.0 10−1

++

++

  1. The contribution to secretion (+) and uptake (−) from either RBCs or the parasite is qualitatively indicated in the last two columns by increasing number of +/− signs from low to high or none ○
  2. C t=0, medium concentration at t = 0; ΔCtrt=48 h, change in concentration at t = 48 h for the uninfected control RBC culture; ΔInft=48 h, change in concentration at t = 48 h for the P. falciparum infected RBC culture; ΔPf Inf, ΔInft=48 h – ΔCtrt=48 h