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Fig. 1 | BMC Systems Biology

Fig. 1

From: Engineering a synthetic anaerobic respiration for reduction of xylose to xylitol using NADH output of glucose catabolism by Escherichia coli AI21

Fig. 1

Engineering a homoxylitol pathway with an anaerobically active pyruvate dehydrogenase and TCA cycle. a NADH output of aerobic glucose catabolism; b Replacing the promoter of the sdhCDAB-sucABCD operon with an anaerobically functional promoter pflBp6; c Homoxylitol pathway with anaerobically active pyruvate dehydrogenase and TCA cycle but without active pentose phosphate pathway. Symbols: the dashed line in a indicates the step is not active under anaerobic condition; the prohibition sign in c indicates the step was blocked by deletion of the gene; ArcA, aerobic regulator binding box; FNR, anaerobic regulator binding box; pflBp6, a promoter of pflB (pyruvate formate-lyase). Genes and abbreviations: PDH, pyruvate dehydrogenase complex; TCA, tricarboxylic acid cycle; sdhCDAB-sucABCD operon, an eight gene operon that encodes for three enzymes: succinate dehydrogenase (sdhCDAB), the α-ketoglutarate dehydrogenase complex (sucAB), and succinyl-CoA synthetase (sucCD); xylA, xylose isomerase gene; xylB, xylulose kinase gene

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BMC Systems Biology

ISSN: 1752-0509

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