From: Flux-sum analysis identifies metabolite targets for strain improvement
Objective | Target metabolites | Flux-sum perturbation | Experimental validation |
---|---|---|---|
Enhance ethanol production | Acetate | Attenuation | |
 | Acetylphosphate | Attenuation | |
 | Formate | Attenuation | N.A. |
 | 6-Phospho-D-gluconate | Intensification | N.A. |
 | Erythrose 4-phosphate | Intensification | N.A. |
 | 5,10-Methylenetetrahydrofolate | Intensification | N.A. |
 | Sedoheptulose 7-phosphate | Intensification | N.A. |
Enhance succinate production | Pyruvate | Attenuation | [11] |
 | Glyoxylate | Intensification | |
 | Menaquinone/menaquinol | Intensification | N.A. |
Minimize acetate production | Acetate | Attenuation | |
 | Acetylphosphate | Attenuation | |
 | 3-Phosphoglycerate | Intensification | N.A. |
 | 4-Methyl-2-oxopentanoate | Intensification | N.A. |
 | Isocitrate | Intensification | [36] |
 | Lactate | Intensification | [34] |
 | Ribulose 5-phosphate | Intensification | N.A. |
 | Succinic semialdehyde | Intensification | N.A. |