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Figure 4 | BMC Systems Biology

Figure 4

From: Spatially-resolved metabolic cooperativity within dense bacterial colonies

Figure 4

Comparison of experimental acetate-associated reporter expression with predicted acetate consumption. (A) Brightfield image of a representative colony expressing GFP under the colntrol of the aceB promoter. This image was taken approximately 48 hours after innoculation when the colony was approximately 2.0 mm in diameter. (B) PaceB-gfp fluorescence in the same colony imaged 100 μm above and parallel to the agar surface. (C) PaceB-gfp fluorescence imaged 300 μm above the agar surface. (D) PaceB-gfp fluorescence in a plane bisecting the colony and perpendicular to the agar surface; this was reconstructed from the entire compiled Z-stack of fluorescence images. (E) Gray-scale plot of the height of a simulated colony. This image was produced after approximately 40 hours of simulation time when the colony was around 2.0 mm in diameter. (F) Predicted acetate uptake rate imaged 100 μm above and parallel to the agar surface. (G) Predicted acetate uptake rate imaged 300 μm above the agar surface. (H) Predicted acetate uptake rate in A plane bisecting the simulated colony and perpendicular to the agar surface.

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