Figure 3

Heatmap of genes in putative drug-resistant cross-talks in breast cancer cell-line: SKBR3 (GSE38376). Heatmap image of comparative gene expression changes of parental and resistant conditions in (A) all 67 genes in all 104, 188 and 299 putative drug-resistant cross-talks using signaling pathways from Reactome, KEGG and WikiPathway database, respectively, and (B) 28 selected genes based on their differential regulation. Here, for each gene, the expression value at each of the 6 conditions (3 parental conditions, and 3 resistant conditions) is the average value of 3 sample patients [17]. For each gene, these 6 expression values (each of them is the average of 3 samples) were transformed into z-scores (zero mean, unit standard deviation) and each z-score was normalized with the maximum absolute value of the z-scores across that particular gene. Note, (B) includes only those genes which belonged to gene-pairs for which the average of GE values at resistant conditions was greater than the average of GE values at parental conditions. For both (A) and (B), red and green bars indicate up-regulation and down-regulation, respectively.