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Table 2 Comparison of experimental observations with simulation

From: Simulations of stressosome activation emphasize allosteric interactions between RsbR and RsbT

Experiment

Phenotype

Reference

Model adaptation

Simulation

reduced RsbX

increase in β-Gal response

[25]

decrease in kdps

increase in post-stress RsbS-P

ΔRsbR(AB), ΔRsbR(ABC), ΔRsbR(ABD)

alteration in pre- and post-stress β-Gal response

[13]

increase in kphs

increase in RsbS-P

ΔRsbR(ABC), RsbRD stressosome

hyperbolic response

[14]

increase in background phosphorylation (allost. par.) decrease of cooperativity

hyperbolic response

RsbR, RsbS phosphorylation after stimulus

transient increase in phosphorylation level

[20]

increase in kphr

increase of RsbR-P

stimulation with different stress level

sigmoidal dose–response curve

[10]

adaptation of the allosteric parameter for R1 and R2 neighbours

allosteric activation of RsbT by RsbR-P

RsbRA T86A, N129A, Q142A, etc.

elevated basal β-Gal level but wild type stress response

[31, 32]

increase of pre-stress kphr

increase of pre-stress RsbR-P

RsbRA L141A, Q147A, L149R

reduced basal β-Gal level but wild type stress response

[32]

decrease of pre-stress kphr

decrease of pre-stress RsbR-P

  1. The phosphorylation of RsbS is correlated to the β-galactosidase response because phosphorylated RsbS releases RsbT, the activator of σB.