From: Simulations of stressosome activation emphasize allosteric interactions between RsbR and RsbT
Experiment | Phenotype | Reference | Model adaptation | Simulation |
---|---|---|---|---|
reduced RsbX | increase in β-Gal response | [25] | decrease in kdps | increase in post-stress RsbS-P |
ΔRsbR(AB), ΔRsbR(ABC), ΔRsbR(ABD) | alteration in pre- and post-stress β-Gal response | [13] | increase in kphs | increase in RsbS-P |
ΔRsbR(ABC), RsbRD stressosome | hyperbolic response | [14] | increase in background phosphorylation (allost. par.) decrease of cooperativity | hyperbolic response |
RsbR, RsbS phosphorylation after stimulus | transient increase in phosphorylation level | [20] | increase in kphr | increase of RsbR-P |
stimulation with different stress level | sigmoidal dose–response curve | [10] | adaptation of the allosteric parameter for R1 and R2 neighbours | allosteric activation of RsbT by RsbR-P |
RsbRA T86A, N129A, Q142A, etc. | elevated basal β-Gal level but wild type stress response | increase of pre-stress kphr | increase of pre-stress RsbR-P | |
RsbRA L141A, Q147A, L149R | reduced basal β-Gal level but wild type stress response | [32] | decrease of pre-stress kphr | decrease of pre-stress RsbR-P |