Figure 2

Thermodynamic analysis of the P. pastoris reaction network, (a) Thermodynamically feasible concentration range of the measured metabolites and expected ranges of the non measured ones. The white bars represent a priori considered metabolite ranges, the light grey bars (measured metabolites) and the green bars (unmeasured metabolites) show the corrected values after performing a network-embedded thermodynamic (NET) analysis. In case of detection of a significant metabolite quantification error, the original measurement (red bar) and concentration ranges before and after the NET analysis are shown together. (b) Transformed Gibbs energy of the cytosolic reactions of the central carbon metabolism of P. pastoris growing on glucose-methanol.