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Figure 1 | BMC Systems Biology

Figure 1

From: Carbon catabolite repression correlates with the maintenance of near invariant molecular crowding in proliferating E. coli cells

Figure 1

E. coli batch cultures in mixed carbon- and single carbon limited media. Each carbon substrate concentration was at 0.2% in single substrate cultures and 0.04% in the mixed substrate experiment. A. Culture growth rates as measured by tracing OD600; B. Relative cell growth = ln(OD600t – OD600t-1); C. measured acetic acid secretion rates; D, E. substrate consumption rates in D./ mixed substrate culture and E./ single substrate cultures; F. the FBAwMC modeling prediction of substrate consumption kinetics in single substrate culture conditions. G, H, The substrate consumption curves (D and E) were normalized to the OD600nm data (A) in individual culture conditions (shown by the dotted points). Curve fitting with one phase decay equation was applied to the normalized data in (G) mixed substrate culture and (H) single substrate cultures (shown in the continuous lines). K is the rate constant and a higher K value indicates a faster substrate uptake. Half-life shows the time points when the normalized substrates concentration decreases to 50%. The data for the black tracings in panels A, B, and the data for panel D are from Ref. [7]).

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