Figure 2

MM127 melanoma cells express mesenchymal markers. Immunofluorescence was used to examine expression of E–cadherin, a cell–to–cell adhesion protein uniquely expressed by epithelial cells (A), N–cadherin, a cell–to–cell adhesion protein uniquely expressed by mesenchymal cells (B), and vimentin, a protein that is uniquely expressed by mesenchymal cells (C) in MM127 cells. The scale bar corresponds to 25 μ m. MM127 melanoma cells were cultured in a circular barrier assay for t=48 hours on glass coverslips in 500 μ L cell medium. All sections were stained with DAPI (blue) to identify the cell nucleus. N–cadherin and vimentin expression are indicated by the green and red staining, respectively. Western blot was used to examine the expression of E–cadherin protein in MM127 cells (Inset in A).