# Table 4 Constrained alignment translation error. Constraining the search to rotations around a single axis. Subtomogram alignment error in terms of the difference in the Euclidean distance between determined and true subtomogram translations. Shown are the medians and median absolute deviations of all 100 subtomogram alignments.

60° angle interval, RCS
SNR 1 0.5 1
Tilt
±90°   0.018 ± 0.0053 0.047 ± 0.017 0.08 ± 0.023 0.28 ± 0.1
±80°   0.027 ± 0.011 0.069 ± 0.029 0.1 ± 0.048 0.37 ± 0.17
±70°   0.037 ± 0.017 0.085 ± 0.04 0.13 ± 0.059 0.45 ± 0.28
± 60°   0.055 ± 0.028 0.14 ± 0.083 0.19 ± 0.11 0.59 ± 0.36
±50°   0.1 ± 0.067 0.18 ± 0.12 0.24 ± 0.12 0.74 ± 0.42
±40°   0.27 ± 0.25 0.49 ± 0.4 0.94 ± 0.83 3.3 ± 2.3
60° angle interval, FCS
SNR 1 0.5 0.1
Tilt
±90°   0.018 ± 0.0062 0.05 ± 0.017 0.074 ± 0.021 0.23 ± 0.097
±80°   0.027 ± 0.013 0.063 ± 0.025 0.076 ± 0.029 0.24 ± 0.091
±70°   0.034 ± 0.015 0.077 ± 0.032 0.098 ± 0.036 0.32 ± 0.16
±60°   0.055 ± 0.033 0.12 ± 0.063 0.18 ± 0.099 0.46 ± 0.28
±50°   0.11 ± 0.075 0.16 ± 0.099 0.2 ± 0.11 0.6 ± 0.36
±40°   0.39 ± 0.35 0.36 ± 0.27 0.57 ± 0.44 3.5 ± 2.8
45° angle interval, RCS
SNR 1 0.5 0.1
Tilt
±90°   0.015 ± 0.005 0.054 ± 0.014 0.063 ± 0.019 0.24 ± 0.086
±80°   0.024 ± 0.0072 0.056 ± 0.018 0.086 ± 0.028 0.29 ± 0.12
±70°   0.033 ± 0.0095 0.075 ± 0.028 0.11 ± 0.04 0.38 ± 0.17
±60°   0.046 ± 0.019 0.1 ± 0.036 0.16 ± 0.051 0.48 ± 0.22
±50°   0.067 ± 0.035 0.14 ± 0.06 0.22 ± 0.098 0.59 ± 0.27
±40°   0.22 ± 0.18 0.31 ± 0.21 0.44 ± 0.29 2.3 ± 1.7
45° angle interval, FCS
SNR 1 0.5 0.1
Tilt
±90°   0.017 ± 0.0053 0.048 ± 0.014 0.06 ± 0.016 0.21 ± 0.074
±80°   0.021 ± 0.0069 0.052 ± 0.018 0.073 ± 0.025 0.2 ± 0.067
±70°   0.03 ± 0.011 0.065 ± 0.023 0.098 ± 0.03 0.26 ± 0.085
±60°   0.043 ± 0.017 0.088 ± 0.033 0.13 ± 0.044 0.32 ± 0.11
±50°   0.07 ± 0.032 0.14 ± 0.056 0.18 ± 0.073 0.44 ± 0.19
±40°   0.17 ± 0.11 0.24 ± 0.16 0.33 ± 0.18 1.5 ± 1.2