Table 3 Constrained alignment rotation error. Constraining the search to rotations around a single axis. Subtomogram alignment error in terms of the difference in the determined and true rotational angle. Shown are the medians and median absolute deviations of all 100 subtomogram alignments. Bold font shows all the alignments with errors larger than 5°, which are considered inaccurate.

∞

1

0.5

0.1

∞

1

0.5

0.1

Tilt

±90°

0.2 ± 0.14

0.31 ± 0.15

0.55 ± 0.26

4.1 ± 1.8

0.21 ± 0.13

0.44 ± 0.24

0.62 ± 0.33

3.1 ± 1.7

±80°

0.29 ± 0.19

0.55 ± 0.38

0.89 ± 0.62

4.5 ± 3

0.44 ± 0.29

0.67 ± 0.41

1 ± 0.73

3.2 ± 1.6

±70°

0.43 ± 0.25

0.67 ± 0.38

0.81 ± 0.54

5.4 ± 3.9

0.57 ± 0.37

0.85 ± 0.53

0.84 ± 0.43

3.8 ± 2.4

±60°

0.6 ± 0.4

0.99 ± 0.79

1.5 ± 1.2

5.9 ± 4.9

0.81 ± 0.64

1.3 ± 1.1

1.7 ± 1.3

3.8 ± 3

±50°

1.1 ± 0.93

1.3 ± 1.1

1.4 ± 0.94

5 ± 4.1

1.7 ± 1.4

1.7 ± 1.4

2 ± 1.3

4.1 ± 3.6

±40°

1.7 ± 1.7

2.3 ± 2.2

7 ± 6.9

42 ± 38

3.9 ± 3.7

3.1 ± 2.9

4 ± 3.6

42 ± 39

∞

1

0.5

0.1

∞

1

0.5

0.1

Tilt

±90°

0.2 ± 0.12

0.35 ± 0.16

0.42 ± 0.25

3.1 ± 1.7

0.19 ± 0.12

0.38 ± 0.19

0.45 ± 0.28

2.5 ± 1.2

±80°

0.18 ± 0.12

0.31 ± 0.21

0.61 ± 0.3

3.9 ± 1.7

0.35 ± 0.23

0.5 ± 0.34

0.5 ± 0.36

2.5 ± 1.4

±70°

0.28 ± 0.15

0.47 ± 0.29

0.56 ± 0.31

3.9 ± 2.1

0.5 ± 0.34

0.64 ± 0.43

0.63 ± 0.45

2.8 ± 1.7

±60°

0.43 ± 0.23

0.49 ± 0.28

0.72 ± 0.37

4.5 ± 3

0.67 ± 0.46

0.64 ± 0.39

0.92 ± 0.46

2.7 ± 1.7

±50°

0.65 ± 0.41

0.89 ± 0.53

0.93 ± 0.64

5.2 ± 3.4

0.99 ± 0.67

1.1 ± 0.72

1.1 ± 0.81

3.1 ± 2.2

±40°

0.98 ± 0.87

1.2 ± 0.9

2 ± 1.7

12 ± 11

1.6 ± 1.2

1.7 ± 1.3

1.6 ± 1.2

9.6 ± 9.2