Table 1 Operating conditions of the fed-batch experiments
From: Cell functional enviromics: Unravelling the function of environmental factors
Fed-batch 1 | Fed-batch 2 | Fed-batch 3 | Fed-batch 4 | Fed-batch 5 | ||
|---|---|---|---|---|---|---|
Initial concentration (mM) | Glc i | 5.5 | 5.7 | 0.18 | 6.2 | 4.8 |
Gln i | 0.2 | 0.5 | 0.42 | 3.9 | 0.1 | |
Glu i | 1.6 | 0.6 | 0.82 | 0.6 | 4.0 | |
Ser i | 2.3 | 1.3 | 1.30 | 1.4 | 1.4 | |
Asp i | 1.4 | 0.8 | 0.65 | 0.8 | 0.8 | |
First feed Started when Glc and/or Gln went below 0.15 mM | Glc = 183.0 Gln = 15.0 Glu = 20.0 | Glc = 182.0 Gln = 5.2 Glu = 31.0 | Glc = 150.0 Gln = 4.2 Glu = 29.2 | Glc = 198.0 | Glc = 183.0 | |
Composition of feeds (mM) | Second feed Started when Ser became limiting (~75 h of operation) | - | Glc = 193.0 Gln = 4.5 Glu = 36.0 Ser = 33.0 Cys = 8.5 | Glc = 160.0 Gln = 5.4 Glu = 25.6 Culture medium 5 times concentrated | Glc = 156.0 Gln = 42.8 | - |
Flow rate | Adjusted to keep glucose between 0.05-0.15 mM or/and glutamine between 0.05-0.25 mM | |||||