Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

Table 3 Predictive power of the hybrid MFA-PLS structure compared to MFA

Experiment #	Productivity^a	MFA prediction	Hybrid MFA prediction
1	1420.7	28915535.7	1214.6^c
6	644.2	-15477747.1	389.9^c/586.4^b
7	920.5	3995861.2	665.7^c/625.6^b
8	462.4	18837398.3	637.7^b
9	455.2	9971383.5	353.5^b
13	3.1	-7199746.4	-130.8^b

^aMeasured specific viral synthesis rate (10³ infectious particles × (10⁶ cells × h)^-1).
^bValidation strategy 1.
^cValidation strategy 2.

ISSN: 1752-0509