DnaA and the timing of chromosome replication in Es-cherichia coli as a function of growth rate

BMC Systems Biology

Table 1 Initial values for the parameters in the model

Parameter	Untransformed Value (at τ= 21 mins)	Units	Reference
Basal transcription rate k_A	75	molecules/min	[70]
RNAP binding $e^{Δ ε_{p d} ∕ k_{B} T}$	12/10000	Dimensionless	[42]
Dna-ATP binding $e^{Δ ε_{a d} ∕ k_{B} T}$	1/10000	Dimensionless	[18], †
RNAP amount P₀	5050	molecules	[41]
RIDA rate k_R	10	molecules/min	[24]
Replication rate k_Λ	1/40	genome equivalents/min	[8]
non-specific binding sites N_NS	5 × 10⁶	(genome equivalent)^-1	[42]

The parameters are fixed with the values in the table for τ = 21 mins and then are able to vary for the other values of τ in order to fix the ratio of DnaA-ATP:chromosome length at the moment of initiation (t = X). †: Chiara Sag-gioro, Anne Olliver, Bianca Sclavi: Multiple levels of regulation in the growth rate dependence of DnaA expression, submitted.

ISSN: 1752-0509