Table 3 Binding constants reported in literature

SHC PTB

EGFR (32 PY peptides)

<2 μM

Protein microarray & SPR^a

[26]

EGFR PY1148 peptide

28 nM

ITC^b

[71]

TRK PY490 peptide

42 nM

SHC SH2

EGFR PY1148 peptide

NB^c

SPR

[27]

EGFR PY1173 peptide

65 nM

PLCγ1 SH2

EGFR PY peptides

<2 μM

Protein microarray

[26]

PLCγ1 N+C SH2

EGFR PY992

200 nM

SPR

[28]

EGFR PY1173

740 nM

STAT1,3 SH2

EGFR PY peptides

>2 μM

Protein microarray

[26]

Grb2 SH2

EGFR PY1068 peptide

30 nM

SPR

[27]

EGFR PY1086 peptide

60 nM

EGFRPY1148 peptide

NB

EGFR PY1173-7Y4

NB

EGFR PY992-8Y4

NB

EGFR phosphopeptides

<2 μM

Protein microarray

[26]

Grb2 protein

EGFR PY1068 peptide

380 nM

ITC, SPR

[31]

EGFR PY1068 peptide

713 nM

SPR

[29]

Activated EGFR in A431 membranes (fractional)

97-650 nM

0.016/nM/s (78%)

7.5-8.1/s (89%)

Single molecule analysis

[32]

0.005/nM/s (21%)

1.6-2.6/s (<11%)

0.0022/nM/s (1%)

0.07-0.4/s (<5%)

p85 N-SH2

PDGFR PY315 peptide

300 nM

ITC, SPR

[72]

p85 2 SH2

IRS-1 PY

0.3-3.0 nM

0.03-0.4/nM/s

0.11-0.19/s

SPR

[73]

Src SH2

(YMXM) peptides polyoma middle tumor antigen

600 nM

ITC

[74]

Lck SH2

Lck Y505 peptide

4 μM

ITC, SPR

[72]

EGFR C-terminus

5 μM

ITC

[74]