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Figure 2 | BMC Systems Biology

Figure 2

From: Spatio-temporal modeling of signaling protein recruitment to EGFR

Figure 2

Analysis and simulation of EGFR tyrosine residues phosphorylation kinetics. A431 cells were serum-starved and treated with batimastat for "resting" condition shown in (A), or treated thereafter for 2 min with 20 nM EGF (B). "Rip-Flips" were prepared and membranes immunogold-labeled with anti-EGFR antibodies. Inset in both (A) and (B) confirms EGFR clustering by Hospkins test. Bars, 0.1 μm. Western blotting method was used to analyze phosphorylation kinetics of EGFR tyrosine residues (C) Y992, (D) Y1068, (E) Y1173, and (G) Y1148. Bands were quantified by densitometry and plotted as density of the bands. (F, H) Results of simulations (dashed lines) agree well with the "fast" kinetics and "slow" kinetics data (solid lines), using parameter values estimated by fitting to the data.

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