Table 2 Predicted essential genes and potential drug targets.

a) Intersection of our predictions with the experimental screen

STM0123

murE

6.3.2.13

UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase

STM0128

murG

2.4.1.227

N-acetylglucosaminyl transferase

STM0129

murC

6.3.2.8

UDP-N-acetylmuramate-L-alanine ligase

STM0154

lpdA

1.8.1.4

Dihydrolipoamide dehydrogenase

STM0218

pyrH

2.7.4.22

Uridylate kinase

STM0221

uppS

2.5.1.31

Undecaprenyl pyrophosphate synthase

STM0222

cdsA

2.7.7.41

CDP-diglyceride synthase

STM0228

lpxA

2.3.1.129

UDP-N-acetylglucosamine acyltransferase

STM0232

accA

6.4.1.2

Acetyl-CoA carboxylase

STM0489

hemH

4.99.1.1

Ferrochelatase

STM0535

lpxH

UDP-2,3-diacylglucosamine hydrolase

STM0542

folD

1.5.1.5, 3.5.4.9

Bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase

STM0988

kdsB

2.7.7.38

CTP:CMP-KDO cytidylyltransferase

STM1194

fabD

2.3.1.39

Acyl carrier protein S-malonyltransferase

STM1195

fabG

1.1.1.100

3-ketoacyl-(acyl-carrier-protein) reductase

STM1200

tmk

2.7.4.9

Thymidylate kinase

STM1700

fabI

1.3.1.10

Enoyl-(acyl carrier protein) reductase

STM2483

dapE

3.5.1.18

Succinyl-diaminopimelate desuccinylase

STM2652

pssA

2.7.8.8

Phosphatidylserine synthase

STM3090

metK

2.5.1.6

STM3415

rpoA

2.7.7.6

DNA-directed RNA polymerase subunit alpha

STM3724

kdtA

3-deoxy-D-manno-octulosonic-acid transferase

STM3730

dfp

4.1.1.36

Pantothenate kinase

STM3912

rep

3.6.1.-

ATP-dependent DNA helicase Rep

STM3978

yigC

3-octaprenyl-4-hydroxybenzoate decarboxylase

STM4153

rpoB

2.7.7.6

DNA-directed RNA polymerase subunit beta

STM4154

rpoC

2.7.7.6

DNA-directed RNA polymerase subunit beta'

b) Predictions for the non-mevalonate pathway

STM0049

ispH, lytB

1.17.1.2

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

STM0220

dxr

1.1.1.267

1-deoxy-D-xylulose 5-phosphate reductoisomerase

STM0422

dxs

2.2.1.7

1-deoxy-D-xylulose-5-phosphate synthase

STM0423

ispA

2.5.1.10

geranyltranstransferase

STM1779

ispE, ipk

2.7.1.149

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

STM2523

ispG, gcpE

1.17.7.1

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

STM2929

ispF

4.6.1.12

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

STM2930

ispD

2.7.7.60

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase