Development and analysis of an in vivo-compatible metabolic network of Mycobacterium tuberculosis

BMC Systems Biology

Table 5 Flux ranges for overall hydrogen ion (H+) exchanges and other related reactions.

Network	Strain	Overall H⁺exchange (mmol/h/gDW)		Nitrite reductase flux (mmol/h/gDW)		Urease flux (mmol/h/gDW)
		Min	Max	Min	Max	Min	Max
iNJ 661v	Wild type	-1.99	-1.93	1.00	1.00	0.00	0.00
	ΔNR	0.04	0.07	0.00	0.00	0.00	0.00
iNJ 661v	Wild type	-3.96	-3.90	1.00	1.00	0.99	1.00
with urea uptake	ΔNR	-1.94	-1.90	0.00	0.00	0.99	1.00
iNJ 661m	Wild type	2.36	3.09	0.00	0.00	0.00	0.00
	ΔNR	2.36	3.09	0.00	0.00	0.00	0.00
iNJ 661m	Wild type	0.43	0.91	0.00	0.00	0.99	1.00
with urea uptake	ΔNR	0.43	0.91	0.00	0.00	0.99	1.00

Overall hydrogen ion (H⁺) exchange indicates the total H⁺ exchange between the environment and the Mycobacterium tuberculosis cells. A negative value of the exchange indicates that M. tuberculosis consumes H⁺ as a whole, while a positive value indicates that the metabolism generate a H⁺ surplus, i.e., increases acidification of the environment. The minimum (Min) and maximum (Max) fluxes reflected the ranges of the fluxes at the optimal growth and were obtained through flux variability analyses of iNJ 661v and iNJ 661 m. ΔNR represents the strain in which nitrite reductase (NR) was blocked. The unit of mmol/h/gDW represents mmol per hour per gram dry weight of M. tuberculosis.

ISSN: 1752-0509