Reconstruction and analysis of genome-scale metabolic model of a photosynthetic bacterium

BMC Systems Biology

Table 4 Comparison of selected fluxes across different growth conditions.

*Reaction* *name*	*Autotrophy*	*Minimum flux*	*Maximum flux*	*Mixotrophy*	*Minimum flux*	*Maximum flux*	*Dark Heterotrophy*	*Minimum flux*	*Maximum flux*	*Light Heterotrophy*	*Minimum flux*	*Maximum flux*	*Reaction description*
2.7.1.2a	0	0	0	0.567	0.566	0.567	0.567	0.566	0.567	0.567	0.566	0.567	beta-D-glucose + ATP → beta-D-glucose-6-phosphate + ADP
4.2.1.2	12.67	12.667	+∞	14.67	14.657	+∞	0.905	0.884	+∞	2.148	1.836	+∞	malate ↔ fumarate + H2O
5.3.1.6	1.201	1.2	+∞	1.269	1.269	+∞	-0.054	-0.051	-0.055	0.066	0.067	+∞	D-ribose-5-phosphate ↔ D-ribulose-5-phosphate
_UQ	0.8	0	0.8	0.8	0	0.8	0	0	0	0.8	0	0.8	PSII* + UQ + 2 H+ → PSII + UQH2
_1.6.5.3	0	0	+∞	0	0	+∞	2.134	0	+∞	0	0	+∞	NADH + UQ + 7 H+ → NAD+ + UQH2 + 4 H+_peribac
_3.6.3.14	38.348	15.7	+∞	21.727	21.7	+∞	4.98	4.95	+∞	6.292	6.281	+∞	3 H+_peribac + phosphate O4P + ADP ↔ 3 H+ + H2O + ATP
6.2.1.1	0.008	-∞	+∞	-30.017	-∞	+∞	-2.124	-∞	+∞	-4.635	-∞	+∞	coenzyme A + acetate + ATP ↔ acetyl-CoA + diphosphate + AMP

Units in mmol g_DW^-1 h^-1. 2.7.1.2a, glucokinase, is the reaction that phosphorylates beta-D-glucose upon entrance in the cell, marking the start of the glycolysis. The flux direction changes can be seen in reaction 4.2.1.2, fumarate hydratase, from TCA cycle and 5.3.1.6, ribose-5-phosphate isomerase, from the pentose phosphate pathway. _UQ and _1.6.5.3 are reactions that reduce UQH2 from photosystem II or NADH oxidation, respectively, causing a pumping of protons to the thylakoid. _3.6.3.14 is the ATP synthase that forms ATP shuttling protons from the thylakoid to the cytosol. 6.2.1.1, acetate-CoA ligase, is the reaction that generates acetyl-CoA from acetate and coenzyme A, that would be a major flux hub in an ethanol-producing strain, standing as the first step of fermentation.

ISSN: 1752-0509