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Table 3 Results on gene essentiality predictions by the finalized mouse model.

From: A detailed genome-wide reconstruction of mouse metabolism based on human Recon 1

Gene Name

Reactions

Subgroup

Mutation lethal

Comment

Reference

EBP

3-beta-hydroxysteroid-delta(8),delta(7)-isomerase

Cholesterol Metabolism

yes

X-linked genes, homozygous males are non-viable (prenatal lethality)

Means et al.

DHCR7

7-dehydrocholesterol reductase

Cholesterol Metabolism

yes

Prenatal lethality of homozygotes

Yu et al.

DHCR24

24-dehydrocholesterol reductase

Cholesterol Metabolism

yes

Prenatal lethality of homozygotes

Mirza et al.

FDFT1

Squalene synthase

Cholesterol Metabolism

yes

Prenatal lethality of homozygotes

Tozawa et al.

HSD17B4

C-3 sterol keto reductase, Beta oxidation of long chain fatty acid, 3-hydroxyacyl-CoA dehydrogenase, hydroxysteroid (17-beta) dehydrogenase 4, peroxisomal lumped long chain fatty acid oxidation

Cholesterol Metabolism

yes

Pre/Peri/Postnathal lethality of homozygotes

Huyghe et al.

NSDHL

C-3 sterol dehydrogenase, C-4 methyl sterol oxidase

Cholesterol Metabolism

yes

X-linked gene, males and homozygous females are non-viable (prenatal lethality)

Cunningham et al

SC5DL

Lathosterol oxidase

Cholesterol Metabolism

yes

Perinatal lethality of homozygotes

Krakowiak et al.

SPTLC1

serine palmitoyltransferase, long chain base subunit 1

Sphingolipid Metabolism

yes

Embryonic lethality of homozygotes

Hojjati et al.

DHFR

dihydrofolate reductase, folate reductase

Folate Metabolism

yes

Embryonic lethality of homozygotes

Di Pietro et al.

PISD

phosphatidylserine decarboxylase, mitochondrial

Glycerophospholipid Metabolism

yes

Embryonic lethality of homozygotes

Steenbergen et al.

PHGDH

phosphoglycerate dehydrogenase

Glycine, Serine, and Threonine Metabolism

yes

Embryonic lethality of homozygotes

Yoshida et al.

HMGCR

Hydroxymethylglutaryl CoA reductase (ir)

Cholesterol Metabolism

yes

Embryonic lethality of homozygotes

Tanaka et al.

CBS

cystathionine beta-synthase, selanocystathionine beta-synthase

Methionine Metabolism

yes

Homozygous mice die within 5 weeks after birth

Watanabe et al.

SPTLC2

serine C-palmitoyltransferase

Sphingolipid Metabolism

yes

Embryonic lethality of homozygotes

Hojjati et al.

PAH

L-Phenylalanine,tetrahydrobiopterin:oxygen oxidoreductase

Tyr, Phe, Trp Biosynthesis

no

Homozygous mice with dysruptions in the gene are viable

http://www.informatics.jax.org

TM7SF2

C-14 sterol reductase

Cholesterol Metabolism

no

Although a mixture of mutations can be lethal, homozygotes for mutations are viable

http://www.informatics.jax.org

Gpam

glycerol-3-phosphate acyltransferase

Triacylglycerol Synthesis

no

Homozygous mice are viable

Howerton et al.