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Table 2 Dimensionless parameter values used for the Full Model.

From: Hypoxia-dependent sequestration of an oxygen sensor by a widespread structural motif can shape the hypoxic response - a predictive kinetic model

Number

Parameter description

Parameter

Values

Source

1

PHD concentration

P ^ t o t

0.2

estimate

2

Dissociation constant of PHD/HIFα binding

K ^ D P

1

[29]

3

(k cat for ODD-hydroxylation)/(basal HIFα degradation rate constant)

k c a t P

500

[31]

4

FIH concentration

F ^ t o t

1

estimate

5

Dissociation constant of FIH/HIFα binding

K ^ D F H

1

set to same as 2

6

(k cat for HIFα CAD-hydroxylation)/(k cat for HIFα ODD-hydroxylation)

ω = K c a t F H K c a t F A

1

estimate

7

(KM of FIH for oxygen)/(KM of PHD for oxygen)

α = K M F K M P

0.33

[30]

8

total concentration of FIH target ARs

A ^ t o t

0 - 500

varied

9

Dissociation constant of FIH/ARD protein binding (unhydroxylated ARs)

K ^ D F A

1

set to same as 2

10

(FIH affinity for hydroxylated)/(FIH affinity for unhydroxylated ARs)

γ

0 - 0.1

varied

11

(basal HIFα degradation rate constant)/(AR degradation rate constant)

ε = k d H k d A

1 - 10

varied

12

Dissociation constant of HIF/HRE binding

K ^ D H R E

0.3

estimate

13

Oxygen concentration

O ˜ 2

0 - 1

varied