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Table 3 Simulated metabolic fluxes corresponding to the best fit presented in Table 2.

From: Edelfosine-induced metabolic changes in cancer cells that precede the overproduction of reactive oxygen species and apoptosis

flux

Control

Edelf: 0.5 μg/mL

Edelf:1 μg/mL

hk***

0.0901118

1

0.12

1

0.135

1

pfk

0.0968532

1.07481

0.120985

1.00821

0.135572

1.004237

fbpase

0.00716729

0.07954

1.46E-3

0.01219

0.0009715

0.007197

pep- > g3p

0.00694225

0.07704

0.00749499

0.06246

0.0025560

0.018933

g3p- > pep

0.186074

2.06492

0.246177

2.05148

0.271442

2.010681

pk

0.181224

2.01110

0.253755

2.11463

0.28068

2.079111

TCA- > pyr

2.23E-3

0.02470

0.0152166

0.12681

0.0118396

0.087701

lac- >

0.12219

1.35598

0.153661

1.28051

0.128901

0.954822

pdh***

0.057984

0.64347

0.0815881

0.67990

0.140089

1.037696

pc

1.05E-3

0.01165

0.0185053

0.15421

0.0116895

0.086589

CitSyn**

0.0351159

0.38969

0.0547404

0.45617

0.120038

0.889170

cit- > mal**

0.0366362

0.40656

0.0549581

0.45798

0.120969

0.896067

PPP*

1.32E-4

1.47E-3

2.80E-6

2.33E-5

3.93E-6

2.91E-5

r5p- >

4.38E-4

0.00486

6.84E-4

0.00570

0.0005799

0.004296

cit- > glt

3.50E-3

0.03884

2.13E-3

0.01775

0.0014090

0.010437

- > cit

0.00549634

0.06099

2.35E-3

0.01959

0.0023398

0.017332

Transketolase:

      

xu5p- > s7p***

0.00826654

0.09174

2.26E-7

1.88E-6

1.46E-6

1.08E-5

s7p- > xu5p

0.0084719

0.09402

4.24E-4

0.00353

0.0003579

0.002651

f6p- > xu5p

5.66E-4

0.00628

3.54E-5

0.00030

3.03E-5

0.000224

xu5p- > f6p***

5.38E-4

0.00597

3.45E-9

2.87E-8

1.89E-8

1.40E-7

f6p- > s7p

0.00534401

0.05930

2.46E-4

0.00205

0.0001979

0.001466

s7p- > f6p

0.0052113

0.05783

4.49E-5

0.00037

3.08E-5

0.000228

xu5p < - > g3p***

8.75E-4

9.71E-3

3.26E-8

2.71E-7

2.21E-7

1.64E-6

f6p < - > e4p

3.48E-4

0.00386

3.75E-6

3.12E-5

2.63E-6

1.95E-5

r5p < - > s7p

0.0800302

0.88812

2.94E-3

0.02453

0.0023626

0.017501

Transaldolase:

      

f6p- > s7p

1.54E-4

0.00171

2.57E-4

0.00214

0.0002003

0.001484

s7p- > f6p

2.48E-5

0.00028

2.25E-5

0.00019

4.54E-6

3.36E-5

f6p < - > g3p

2.64E-7

2.93E-6

1.09E-6

9.05E-6

2.51E-7

1.86E-6

s7p < - > e4p

0.0144573

0.16044

0.0053368

0.04447

0.0037384

0.027691

  1. The fluxes were computed for cells incubated for 48 hours without drugs (control) or treated with either 0.5 μg × mL-1 of edelfosine that caused less than 1% of apoptosis ( 0-1%) or with 1.0 μg × mL-1 of edelfosine that caused 4-5% of apoptotis (4-5%). Left column for each condition shows absolute value of the flux (in mM × min-1 in intracellular volume), right column shows the values normalized per respective glucose uptake (hk). The flux depicted as TCA- > pyr is a sum of fluxes through pepck and malic enzyme, that resulted in the same isotopomer distribution and contribute to the 13C enrichment in lactate from the TCA cycle. Arrows indicate the directions of fluxes; stars indicate the fluxes for which the absolute values (not normalized) are statistically different from control (***, p < 0.01; **, p < 0.05; *, p < 0.1).
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BMC Systems Biology

ISSN: 1752-0509

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