Figure 2
From: Model-based extension of high-throughput to high-content data
Rates and currents of STAT5B nucleocytoplasmic cycling. (A) Summary of all rates α imp and α exp . The rates directly correspond to the exponential of the fits to the FRAP data. Gaussian error propagation leads to the error bars for α imp and α exp . The relative uncertainty of the fitted parameter a1 is negligible compared to the relative uncertainty of the denominator. Relative errors of concentrations and volumes can be estimated to be around 10% and lead to the rate uncertainties. In addition, for the currents (panels (B)-(D)) a small constant error has been added to every point to avoid overvaluing small currents. (B) Michaelis-Menten fit for import (left) and export currents (right) not normalized, (C) normalized to nucleus surface area and (D) normalized to the respective originating compartment volume. χ2 values of the fit are indicated in the plot.