Figure 3

Schematic representation and simulation results of the mathematical model of the activation status of PP2Ac at the IKK complex. (A) The IL-1 receptor and IL-1 form the IL-1 receptor complex which triggers IKKβ phosphorylation. PP2Ac dephosphorylates phosphorylated IKKβ and is deactivated by UVB radiation. Ellipses denote model inputs, boxes with solid lines denote state variables, and boxes with dotted lines denote species whose concentration can be calculated from the state variables by mass conservation relations. Normal arrows denote mass flows following mass action kinetics, circles at the arrow tail signify that the involved species participates catalytically in the reaction (signal flow). (B) The model assuming constitutive PP2Ac activity matches the experimental data calculated from Figure 1 well (χ² = 10.46 + 13.99 = 24.45). Experimental data are shown as mean and standard deviation of three independently performed experiments.