Figure 1

Failure of IκBα reappearance coincides with continuous IKKβ phosphorylation and NFκB activation. KB cells were left untreated or stimulated with 10 ng/ml IL-1 alone or in combination with UVB (300 J/m²). At the indicated time points cytosolic as well as nuclear protein extracts were generated. Cytosolic extracts were analysed for the phosphorylation status of IKKβ and IκBα degradation by Western-blotting. Equal loading was monitored with an antibody against α-tubulin. In parallel, nuclear translocation of NFκB was determined by EMSA with an NFκB specific oligonucleotide. Data shown represent one out of three independently performed experiments.