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Table 1 Uptake and production rates and biomass concentration in S. cerevisiae CEN.PK113-1A chemostat cultures. Glucose uptake rate, glycerol, acetate and ethanol production rate and biomass concentration (mean ± SEM) of the 13C-labelled glucose-limited chemostat cultures (D = 0.10 h-1, pH 5.0, 30°C, 1,5 vvm gas flow) of S. cerevisiae CEN.PK113-1A used as input values in the 13C-MFA.

From: Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A

 

O2 provided

 

20.9%

2.8%

1.0%

0.5%

0.0%

 

I

II

I

II

I

II

I

II

I

II

Glucose uptake rate [mmol/(g DW)-1h-1]

1.28 ± 0.04

0.87 ± 0.04

1.36 ± 0.04

1.28 ± 0.02

1.97 ± 0.02

2.13 ± 0.06

2.20 ± 0.09

2.78 ± 0.16

6.30 ± 0.25

6.58 ± 0.16

Glycerol production rate [mmol/(g DW)-1h-1]

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

1.05 ± 0.01

1.11 ± 0.03

Acetate production rate [mmol/(g DW)-1h-1]

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

0.00 ± 0.00

Ethanol production rate [mmol/(g DW)-1h-1]

0.00 ± 0.00

0.00 ± 0.00

0.07 ± 0.00

0.10 ± 0.01

1.56 ± 0.02

2.00 ± 0.08

2.59 ± 0.09

2.91 ± 0.18

9.05 ± 0.21

9.47 ± 0.31

Biomass (g DW l-1)

5.17 ± 0.04

5.31 ± 0.12

4.61 ± 0.09

4.86 ± 0.05

3.22 ± 0.02

2.70 ± 0.03

2.21 ± 0.03

2.08 ± 0.03

1.03 ± 0.02

0.98 ± 0.02