Figure 3

Self-organizing map (SOM) Analysis. A. U-matrix. Measured metabolites (n = 56) were arranged in a 20 × 20 lattice on the basis of diurnal change similarities. Light- and dark shading indicate high and low similarity, respectively. B. Phenotypic linkage map (PLM). The linkage among metabolites based on dynamic similarity is expressed as the distance on the quadratic plane. The metabolites were assigned to 14 metabolic modules that fluctuated synchronously; most contained traditional metabolic pathway networks or similar compounds. M1, major amino acid; M2, related to photorespiratory pathway intermediates; M3, pentose phosphate pathway; M4, latter half of the glycolytic pathway; M5, latter half of the TCA cycle; M6, environmental stress response; M7, sugars; M8, NADH and NADPH; M9, first half of the glycolytic pathway; M10; first half of the TCA cycle; M11, minor amino acids; M12, nucleoside tri- and diphosphates. C. Time-resolved layout. The relative levels of metabolites are shown for every time point from the start of the light period to the end of the dark period. Light and dark shading indicate high and low levels.