Figure 2

dFmr1 loss of function enhances sticky RNAi knockdown phenotypes. Plot of phenotype penetrance for the genotypes: ey-GAL4/+;UAS-sticky RNAi/+ (n = 162), ey-GAL4/UAS-dFmr1 RNAi; UAS-sticky RNAi/+ (n = 36), and ey-GAL4/+;UAS-sticky RNAi/dFmr1³ (n = 32). No phenotypes were observed in flies of the genotype ey-GAL4/UAS-dFmr RNAi (data not shown). Phenotypes included are Rough Eye (RE), Reduced Eye (Red), No Eye (NE), Vibrissae (Vib), Frontorbital bristles (FO), Orbital and Vertical bristles (OV), Postorbital bristles (PO), Frontal bristles (F), Maxillary Palps (MP), and Necrosis (N). For RE, Red, Vib and PO we found statistically significant enhancement in the double RNAi lines when compared to the sticky-RNAi line alone, p < 0.05 in a Fisher's Exact test. This was also true for sticky-RNAi line in a dFmr1³ heterozygous compared to the sticky-RNAi line alone.