Figure 3

Dynamical behavior of Network I. (A) Average number of GFP molecules in the absence of Tc (WT w/o Tc, blue line) and when 2000 molecules (WT/w 2000 Tc, green line) or 5000 molecules (WT/w 2000 Tc, red line) of Tc are added into the medium at time 2 × 10⁴ sec, using wild-type kinetics. (B) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 10⁴ sec, using wild-type kinetics (WT/w 2000 Tc, blue line), a 20 fold (Des 1.1/w 2000 Tc, green line) and a 50 fold (Des 1.2/w 2000 Tc, red line) increase in the dissociation constant of induced Tet-ON from TetO of the gene encoding Tet-ON. (C) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 10⁴ sec and 6 × 10⁴ sec, using wild-type kinetics (WT/w 2000 Tc (x2), blue line), a 20 fold (Des 1.1/w 2000 Tc (x2), green line) increase in the dissociation constant of induced Tet-ON from TetO of the gene encoding Tet-ON. (D) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 10⁴ sec, using wild-type kinetics (WT/w 2000 Tc, blue line), a doubled (Des 1.3/w 2000 Tc, green line) and a quadrupled (Des 1.4/w 2000 Tc, red line) half-life of Tet-ON.