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Table 1 Proteins involved in ISC synthesis in Saccharomyces cerevisiae.

From: In silico pathway reconstruction: Iron-sulfur cluster biogenesis in Saccharomyces cerevisiae

Proteins

Protein Function

Type of evidence for Protein Function

Suggested Function In ISC Assembly

Type of evidence for suggested systemic role

Arh1

Ferredoxin Reductase

Homology to known ferredoxin/adrenodoxin reductases

Reduces Yah1/Provides electrons for ISC assembly/transfer/repair

Homology, Physiological, Genetic

Yah1

Ferredoxin

Homology to known ferredoxin/adrenodoxin

Provides electrons for ISC assembly/transfer/repair

Physiological, Genetic

Yfh1

Frataxin

Homology, Biochemical; Structural

Stores/Provides Fe directly to ISC assembly and Heme synthesis

Physiological, Structural, Genetic

Grx5

Glutaredoxin

Homology, Biochemical

Regulates glutathionylation state of protein cysteinyl residues

Physiological, Biochemical

Isa1

Scaffold

 

As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins

Biochemical, Spectroscopic, Direct transfer observation

Isa2

Scaffold

 

As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins

Biochemical, Spectroscopic, Direct transfer observation

Isu1

Scaffold

 

As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins

Biochemical, Spectroscopic, Direct transfer observation

Isu2

Scaffold

 

As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins

Biochemical, Spectroscopic, Direct transfer observation

Nfu1

Scaffold

 

As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins

Biochemical, Spectroscopic

Ssq1

Hsp70 Chaperone

Homology, Biochemical, Genetic, Structural

Assists in proper folding of ISC biosynthetic proteins, namely Yfh1 and Isa-Isu proteins/Assists in maintaining ISC assembled in scaffold dimer for proper transfer

Biochemical, Structural

Jac1

Hsp40 Co- chaperone

Homology, Biochemical, Genetic, Structural

Assists Ssq1 in interacting with Isu/Isa proteins

Biochemical, Genetic, Structural

Mge1

Co- chaperone/Nucleotide exchange factor

Homology, Biochemical, Genetic, Structural

Assists Ssq1

Biochemical, Genetic, Structural

Nfs1

Cysteine Desulfurase

Homology, Biochemical

Provides sulphur for ISC assembly in scaffold dimers or in situ ISC assembly/repair

Physiological, Biochemical, Genetics

Atm1

ABC transporter

Homology

Involved in ISC export for cytoplasm and nuclear proteins

Physiological, Genetic

Isd11

unknown

Biochemical, Genetic

Fundamental for Nfs1 action

Physiological, Genetic